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KMID : 1134819980270020232
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Journal of the Korean Society of Food Science and Nutrition
1998 Volume.27 No. 2 p.232 ~ p.238
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Antimicrobial Activity of Defatted Camellia(Camellia japonica L.) Seeds Extract
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Kang Seong-Koo
Kim Yong-Doo
Choi Ok-Ja
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Abstract
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To develope natural food preservatives, ethanol and water extracts were prepared from the defatted camellia(Camellia japonica L.) seeds. Antimicrobial activities were examined against 14 microorganisms which were food borne pathogens and/or food poisoning microorganisms and food-related bacteria and yeasts. Ethanol extract showed antimicrobial activities against several microorganisms tested, but not on lactic acid bactria. Especially, minimum inhibitory concentrations(MIC) for yeasts were as low as 1㎎/ml. Water extract exhibited antimicrobial activities for the yeasts tested, but almost bacteria were not observed. The ethanol extract was fractionated in the order of hexane, chloroform, ethylacetate and butanol fractions to test antimicrobial activity. The strongest activity for the tested yeasts were found in the butanol fractions, but bacteria were chloroform fraction. Hansenula anomala treated with ethanol extract were observed by scanning electron micrograph, and the results exhibited morphological changes, including the irregularly contracted cell surface and expanded ellipsoidal shape.
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KEYWORD
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defatted camellia seeds, antimicrobial activity, minimum inhibitory concentration
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